PHYSICOCHEMICAL PROPERTIES OF YOGHURT PROCESSED FROM THE MILK OF COWS FED CLOVE BUD (Syzygium aromaticum) AND BLACK SEED (Nigella sativa) POWDER AS AFFECTED BY STORAGE

Authors

  • Aisha Abdulqadeer Muhammad
    Ahmadu Bello University
  • Shehu Lurwanu Ibrahim
    Bayero University, Kano

Keywords:

Yoghurt, Clove bud powder, Black seed powder, Physicochemical, Antioxidant

Abstract

This study evaluated the physicochemical properties of yoghurt processed from the milk of cows fed clove bud powder (CBP) and black seed powder (BSP). Twelve Romanian spotted dairy cows were divided into four groups: negative control, positive control (synthetic antioxidants), CBP and BSP (1.5 % DM) in a completely randomized design. The cows were fed for 21 days, milk collected and processed into yoghurt. The yoghurts were analysed over 28 days of refrigerated storage. Results showed that pH (4.208 to 4.112) and titratable acidity (1.22 to 0.88 %) decreased, while ash (0.640 to 1.300 %), protein (3.068 to 3.435 %), moisture (89.075 to 94.675 %), and fat content (2.478 to 3.850 %) (p < 0.05) significantly increased with storage days (day 0 to 28, respectively). The study concludes that supplementing cow diets with CBP and BSP improved the nutritional profile offering a natural alternative to synthetic additives. For a more nutrient-dense yoghurt, it is recommended that dairy cows be fed up to 1.5 % DM of clove bud and black seed powders.

Author Biography

Shehu Lurwanu Ibrahim

Department of Animal Science, Senior Lecturer

Dimensions

Abdalla, M. O. M., & Ahmed, S. Z. A. N. (2010). Chemical composition of Mish “A traditional fermented dairy product” from different plants during storage. Pakistan Journal of Nutrition. 9 (3): 209-212.

Abdi-Moghadam, Z., Darroudi, M., Mahmoudzadeh, M., Mohtashami, M., Jamal, A. M., Shamloo, E., & Rezaei, Z. (2023). Functional yoghurt, enriched and probiotic: A focus on human health. The European Society for Clinical Nutrition and Metabolism. 57: 575 – 586. https://doi.10.1016/j.clnesp.2023.08.05

Abdul Hakim, B. N., Xuan, N. J., & and Oslan, S. N. H. (2023). A comprehensive review of bioactive compounds from lactic acid bacteria: potential functions as functional food in dietetic and the food industry. Foods. 12 (15): 2850. https://doi.org/10.3390/foods12152850

Adetoro, O. F., Kajihausa, O. E., Sobukola, O. P., & Afolabi, O. W. A. (2025). Evaluation of high quality cassava flour wheat bread spiced with clove powder. FUDMA Journal of Sciences. 9 (9): 116 – 122.

Ali, G. A. A., El-Dardiry, A. I., & El-Rahmany, A. S. (2022). Study of the physicochemical, rheological, functional, microstructure, microbial and sensory properties of Kareish cheese fortified with germinated quinoa seeds and processed using ultrasound technology. Egyptian Journal of Chemistry. 65 (11): 515 – 529. Doi: https://doi.org/10.21608/EJCHEM.2022.152795.6615

Aljobair, M. O. (2024). Fortification of yoghurt with cloves extract: Quality characteristics, sensory attributes, and antioxidant evaluation. Italian Journal of Food Science. 36 (4): 288–304.

Al Shaikh S. A. H., Alhamid, M. A., & Aldhalemi, A. A. (2020). Study of the effect of fortified milk by zinc salts in different concentrations on the sensory and physicochemical properties of the processed yoghurt. Plant Archives. 20 (2): 81-90.

Al Shaikh, S. A. H., Alhamid, M. A., & Al-Obaidi, L. F. H. (2022). Study of the effect of black seed extract on some physicochemical, microbiological and sensory properties of the manufactured yoghurt. Biochemical Cell Archives. 22 (2): 3583 – 3592. https://connectjournals.com/03896.2022.22.3583

AOAC International. (2000). Official methods of analysis of AOAC International (17th ed.).

Atwaa, E, H., Shahein, M. R., Abd El-Sattar, E., Hijazy, H. H. A., Albrakati, A., & Elmahallawy, E. K. (2022). Bioactivity, physicochemical and sensory properties of probiotic yoghurt made from whole milk powder reconstituted in aqueous fennel extract. Fermentation. 8 (52): 1 – 11.

Bampidis, V., Azimonti, G., Bastos, M. L., Christensen, H., Dusemund, B., Durjava, M. F., Kouba, M., Lopez-Alonso, M., Puente, S. L., Marcon, F., Mayo, B., Pechova, A., Petkova, M., Ramos, F., Sanz, Y., Villa, R. E., Woutersen, R., Finizio, A., Teodorovic, I., … Innocenti, M. (2022). Safety and efficacy of a feed additive consisting of butylated hydroxytoluene (BHT) for all animal species (Katyon Technologies Limited). EFSA Journal. 20 (5): e07287 doi: https://doi.org/10.2903/j.efsa.2022.7287

Bikheet, M. M., Hassan, H. M., Omar, M. O. A., Abdel-Aleem, W. M., Galal, S. M., Korma, S. A., Ibrahim, S. A., & Nassar, K. S. (2025). Effects of clove (Syzygium aromaticum) extract on antibacterial activity, phytochemical properties and storage quality of flavored milk beverages. Journal of Dairy Science. 108: 3300 – 3313. https://doi.org/10.3168/jds.2024-26023

Caleja, C., Barros, L., Antonio, A. L., Carocho, M., Oliveira, M. B. P., & Ferreira, I. C. (2016). Fortification of yoghurts with different antioxidant preservatives: A comparative study between natural and synthetic additives. Food Chemistry. 210: 262–268.

Carlsen, M. H., Halvorsen, B. L., & Holte, K. (2010). The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutrition Journal. 9 (1): 1‒11.

El-Sayed, S. M., & Youssef, A. M. (2019). Potential application of herbs and spices and their effects in functional dairy products. Heliyon. 5 (6): e01989

Ferrando, N., Pino-Otin, M. R., Terrado, E., Ballestero, D., & Langa, E. (2024). Bioactivity of eugenol: a potential antibiotic adjuvant with minimal ecotoxicological impact. International Journal of Molecular Science. 25 (13): 7069. Doi: https://doi.org/10.3390/ijms25137069

Gahruie, H. H., Eskandari, M. H., Mesbahi, G., & Hanifpour, M. A. (2015). Scientific and technical aspects of yoghurt fortification: a review. Food Science and Human Wellness. 4 (1): 1–8.

Gulbas, Y., & Saldamli, I. (2005). The effect of selenium and zinc fortification on the quality of Turkish white cheese. International Journal of Food Science and Nutrition. 56: 141-146.

Hamid, O. I. A., & Abdelrahman, N. A. M. (2012). Effect of adding cardamom, cinnamon and fenugreek to goat’s milk curd on the quality of white cheese during storage. International Journal of Dairy Science. 7: 43–50.

Horlacher, N., Oey, I., & Agyei, D. (2023). Learning from tradition: health-promoting potential of traditional lactic acid fermentation to drive innovation in fermented plant-based dairy alternatives. Fermentation. 9 (5): 452. doi: https://doi.org/10.3390/fermentation9050452

Kabir, Y., Shirakawa, H., & Komai, M. (2019). Nutritional composition of the indigenous cultivar of black cumin seeds from Bangladesh. Progress in Nutrition. 21 (1-S): 428-434.

Karrar, E., Mahdi, A. A., Sheth, S., Mohamed Ahmed, I. A., Manzoor, M. F., Wei, W., & Wang, X. (2022). Nutritional composition and volatile compounds of black cumin (Nigella sativa L.) seed, fatty acid composition and tocopherols, polyphenols and antioxidant activity of its essential oil. Horticulturae. 8 (7): 575

Khalil, O. S. F., Ismail, H. A., & Elkot, W. F. (2022). Physicochemical, functional and sensory properties of probiotic yoghurt flavored with white sapote fruit (Casimiroa edulis). Journal of Food Science and Technology. 59: 3700–3710.

Milind, P., & Deepa, K. (2011). Clove: a champion spice. International Journal of Research in Ayurveda and Pharmacy. 2 (1): 47-54.

Nakov, G., Ninova-Nikolova, N., Ivanova, N., Raykova, V., Trajkovska, B., Colic, M. L., Lukinac, J., & Jukic, M. (2024). Yoghurt Enriched with Chia Seeds: Physicochemical, Microbiological, and Sensory Changes during Storage. Fermentation. 10 (431): 1 – 16.

Nguyen, P. T. M., Kravchuk, O., Bhandari, B., & Prakash, S. (2017). Effects of different hydrocolloids on texture, rheology, tribology and sensory perception of texture and mouthfeel of low-fat pot-set yoghurt. Food Hydrocolloids. 35: 1 – 4. DOI: https://doi.org/10.1016/j.foodhyd.2017.05.035

Nistor, E., Dobrei, A. G., Dobrei, A., & Camen, D. (2018). Growing Season Climate Variability and its Influence on Sauvignon Blanc and Pinot Gris Berries and Wine Quality: Study Case in Romania (2005-2015). South African Journal of Enology and Viticulture. 39 (2): 196 – 207. DOI: https://doi.org/10.21548./39-2-2730

Obeidat, B. S., Al-Khazaleh, J., & Alqudah, A. M. (2023). Black cumin meal (Nigella sativa) as an alternative feed resource during the suckling period of Awassi ewes: Assessments of performance and health. Animal Feed Science and Technology. 306: 115820

Ouazib, M., Benhamdi, A., Akachat, B., & Bounanaa, F. D. (2023). Effects of supplementation with Algerian Nigella sativa bioactive compounds on quality of yoghurt. Food Technology. 47 (2): 95 – 110.

Paskudska, A., Kołodziejczyk, D., & Socha, S. (2018). The use of herbs in animal nutrition. Proceedings of the Polish Society of Animal Science. 17: 3–14.

Radzikowski, D., Milczarek, A., Janocha, A., Ostaszewska, U., & Niedziałek, G. (2020). Feed additives in the diet of high-producing dairy cows. Proceedings of the Polish Society of Animal Science. 19: 5–16.

Sanusi, M. S., Raji, A., Alaka, A., & Abdulazeez, A. (2023). Physicochemical, antioxidant and microbial properties of sweetened yoghurt produced from partial substitution of sugar with soursop puree. Food Production, Processing and Nutrition. 5: 13. https://doi.org/10.1186/s43014-022-00126-9

Stobiecka, M., Krol, J., & Brodziak, A. (2023). Antioxidant Potential of Yoghurts Produced from Milk of Cows Fed Fodder Supplemented with Herbal Mixture with Regard to Refrigerated Storage. Applied Sciences. 13 (10469): 1 – 14. DOI: https://doi.org/10.3390/app131810469

Tang, J., Zhang, W., Yuan, R., Shu, Y., Liu, G., Zheng, B., & Tu, J. (2024). Fortification of yoghurt with mulberry leaf extract: Effects on physicochemical, antioxidant, microbiological and sensory properties during 21-days of storage. Heliyon. 10 e37601. https://doi.org/10.1016/j.heliyon.2024.e37601

Uddin, M. A., Shahinuzzaman, M., Sohel Rana, M., & Yaakob, Z. (2017). Study of chemical composition and medicinal properties of volatile oil from clove buds (Eugenia caryophyllus). International Journal of Pharmaceutical Sciences and Research. 8 (2): 895-899.

Uddin, M. J., Islam, M. R., & Absar, N. (2024). Nutritional profile and physicochemical analysis of two indigenous cultivars of black cumin (Nigella sativa) seeds and oil. Food Research. 8 (2): 1 – 8.

Ulanowska, M., & Olas, B. (2021). Biological Properties and Prospects for the Application of Eugenol—A Review. International Journal of Molecular Science. 22: 3671.

Walkenhorst, M., Leiber, F., Maeschli, A., Kapp, A. N., Spengler-Neff, A., Faleschini, M. T., Garo, E., Hamburger, M., Potterat, O., & Mayer, P. (2020). A multicomponent herbal feed additive improves somatic cell counts in dairy cows—A two stage, multicentre, placebo-controlled long-term on-farm trial. Journal of Animal Physiology and Animal Nutrition. 104: 439–452.

Gross Composition of Supplemented Diet fed to Dairy Cows in Timisoara Farm

Published

27-10-2025

How to Cite

Muhammad, A. A., & Ibrahim, S. L. (2025). PHYSICOCHEMICAL PROPERTIES OF YOGHURT PROCESSED FROM THE MILK OF COWS FED CLOVE BUD (Syzygium aromaticum) AND BLACK SEED (Nigella sativa) POWDER AS AFFECTED BY STORAGE. FUDMA JOURNAL OF SCIENCES, 9(11), 191-195. https://doi.org/10.33003/fjs-2025-0911-3943

How to Cite

Muhammad, A. A., & Ibrahim, S. L. (2025). PHYSICOCHEMICAL PROPERTIES OF YOGHURT PROCESSED FROM THE MILK OF COWS FED CLOVE BUD (Syzygium aromaticum) AND BLACK SEED (Nigella sativa) POWDER AS AFFECTED BY STORAGE. FUDMA JOURNAL OF SCIENCES, 9(11), 191-195. https://doi.org/10.33003/fjs-2025-0911-3943