MOLECULAR PROFILING AND ANTIMICROBIAL RESISTANCE PATTERNS OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI ISOLATES FROM CLINICAL SAMPLES IN SOUTHWESTERN NIGERIA

Authors

  • Abiodun A. Akindele
    Ladoke Akintola University of Technology, Ogbomoso
  • Elizabeth O. Aro
    College of Health Sciences, Ladoke Akintola University of Technology, Ogbomosho, Nigeria.
  • Richard O. Ojedele
    Nigeria Center for Disease Control and Prevention (NCDC)
  • Gbolabo Odewale
    Federal University Lokoja image/svg+xml
  • Oloyede S. Bolaji
    Osun State University image/svg+xml

Keywords:

Molecular characterization, Extended-Spectrum Beta-Lactamases, Escherichia coli clinical isolates, Southwestern Nigeria

Abstract

Introduction: Escherichia coli (E. coli) is a Gram-negative that can become true pathogenic. Extended-spectrum Beta-lactamases (ESBLs) are enzymes that make bacteria resistant to antibiotics, posing risks for infections and requiring strict infection control measures.

Methodology: A hospital-based study in Southwestern Nigeria (August–November 2020) analyzed 105 Escherichia coli isolates using biochemical tests, EMB agar, and PCR for molecular identification. Antibiotic susceptibility was assessed via the Kirby-Bauer method, interpreted using CLSI criteria. Molecular analysis identified blaCTX, blaSHV, and blaTEM genes. Data underwent descriptive analysis and Chi-square testing, with a p-value <0.05 considered significant, highlighting associations between variables and antibiotic resistance genes.

Results: It was discovered that 35 out of 105 (33.3%) clinical Escherichia coli isolates were confirmed to be Escherichia coli isolates using the TEcol primer. At least one of the three (3) genes of ESBL was screened for in 60% of the confirmed Escherichia coli isolates; 17.1%, 37.1% and 40% of the isolates had SHV, TEMH and CTX-M genes respectively. There was 100% resistivity of the extended-spectrum beta-lactamases producing Escherichia coli isolates to Amoxycillin/Clavulanic acid, Meropenem, Oxacillin, Ceftazidime and Cefepime; 33.3%, 47.6%, 61.9%, 71.4% and 81% of the extended-spectrum beta-lactamases-producing isolates were resistant to Imipenem, Chloramphenicol, Levofloxacin, Ciprofloxacin and Cefoxitin respectively.

Significance of Results: There is prevalence of blaCTX-M-positive extended-spectrum beta-lactamases isolates, and increasing spread of extended-spectrum beta-lactamases-producing clinical Escherichia coli isolates in Southwest Nigeria.

Conclusion: There is existence and increasing production of Extended-spectrum Beta-lactamases in some clinical isolates of Escherichia coli. 

Dimensions

Abba, P. O., Umeh, E. U., Gberikon, G. M., & Agbo, E. B. (2019). Emergence of bla TEM resistance gene in ESBL-producing Escherichia coli clinical isolates from Health facilities in Makurdi, Benue State Nigeria. Emergence.

Adekunle, O. C., Falade-Fatila, A. J., & Odewale, G. (2019). Molecular Detection ctx-M, TEM and VIM in ESBL-Producing E. coli Strains Isolated from Pregnant Women in Osogbo. Microbiol Res J Int, 28(2), 1-8.

Adeyemo, A. T., Adeyemo, A. T., Odetoyin, B. W., & Onipede, A. O. (2020). Prevalence and Molecular Characteristics of Extended-Spectrum BetaLactamase-Producing Gram-Negative Pathogens from Patients in a Tertiary Care Hospital in Nigeria. Journal of Medical Science and Clinical Research 8(5), 1-10. https://dx.doi.org/10.18535/jmscr/v8i5.01

Aibinu, I., Aednipekun, E., & Odugbemi, T. (2004). Emergence of Quinolone Resistance amongst Escherichia coli strains isolated from Clinical infections in some Lagos State Hospitals, in Nigeria. Nigerian Journal of Health and Biomedical Sciences, 3(2), 73-78.

Akinbami, O. R., Olofinsae, S., & Ayeni, F. A. (2018). Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia, Morganella morganii, Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria. PeerJ, 6, e5053.

Akneden, A., Trkel, S., & iek, C. (2025). Antimicrobial susceptibility patterns and extended-spectrum -Lactamase production by enterobacterales in a tertiary hospital. Aksaray niversitesi Tp Bilimleri Dergisi, 5(1), 1-6.

Alabi, O., Obisesan, A., Taiwo, M., & Adewuyi, R. (2020). CO-PRODUCTION OF EXTENDED-SPECTRUM BETA-LACTAMASES AND METALLO BETA-LACTAMASES AMONG MULTI-DRUG RESISTANT GRAM-NEGATIVE BACTERIA ISOLATES COLLECTED FROM TERTIARY HOSPITALS IN OYO STATE, NIGERIA. FUDMA JOURNAL OF SCIENCES, 4(3), 230-241.

Ansari, S. K., Asha, G., Kumar, M., Afaq, N., & Verma, R (2025). TO STUDY THE MOLECULAR PROFILING AND ITS ASSOCIATION TO EXTENDED SPECTRUM -LACTAMASE (ESBL) PRODUCING E. coli ISOLATES. SEEJPH, 2197-5248.

Arsalane, L., Zerouali, K., Katfy, K., & Zouhair, S. (2015). Molecular characterization of extended spectrum -lactamase-producing Escherichia coli in a university hospital in Morocco, North Africa. African Journal of Urology, 21(3), 161-166.

Ayalneh, S. T., Beshah, B. Y., Jeon, Y., Wami, A. A., Teshome, S., Gebreselassie, S., ... & Abegaz, W. E. (2025). Genetic profiling of extended-spectrum -Lactamase and carbapenemase-producing Escherichia coli O157: H7 from clinical samples among diarrheal patients in Shashemene, Ethiopia. BMC Infectious Diseases, 25(1), 90.

Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4_ts), 493-496.

Bello, O. O., Oni, M. O., Bello, J. O., & Bello, T. K. (2019). The incidence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in salad vegetables in Ondo City, Nigeria. Asian Food Science Journal, pp.1-14.

Ben Slama, K., Ben Sallem, R., Jouini, A., Rachid, S., Moussa, L., Senz, Y., ... & Torres, C. (2011). Diversity of genetic lineages among CTX-M-15 and CTX-M-14 producing Escherichia coli strains in a Tunisian hospital. Current Microbiology, 62, 1794-1801.

Bradford, P. A. (2001). Extended-spectrum -lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clinical microbiology reviews, 14(4), 933-951.

Brolund, A. (2014). Overview of ESBL-producing Enterobacteriaceae from a Nordic perspective. Infection ecology & epidemiology, 4(1), 24555.

Cheesbrough, M. (2006). District laboratory practice in tropical countries, part 2. Cambridge university press.

Clinical and Laboratory Standards Institute (CLSI) (2017). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard. CLSI Document M100. 27th ed.

Egbule, O. S., & Ejechi, B. O. (2021). Prevalence of extended spectrum beta-lactamases (ESBLS) producing Escherichia coli and Klebsiella pneumoniae among hospitalized patients from nigeria. FUDMA JOURNAL OF SCIENCES, 5(2), 584-595.

Egbule, O. S., Ejechi, B. O., Uwhe-Ureghe, U. B., Okwumabua, O., IWERIEBOR, B. C., & Obi, L. C. (2024). Transformation mediated transfer of Extended Spectrum Beta lactamases (ESBLs) and Sul 1 genes obtained from trimethoprim-sulfamethoxazole resistant (TSR) Escherichia coli and Pseudomonas aeruginosa clinical isolates. Microbes and Infectious Diseases, 5(4), 1450-1460.

Etang, U. E., Moses, A. E., Akpan, S. S., Eyo, A. O., & Moses, E. A. (2025). Predominance of metallo-beta-lactamase blaVIM Genes in Clinical Isolates from Health Facilities in Nigeria: Role in Multidrug Resistance Surge. Ibom Medical Journal, 18(1), 105-113.

Fang, H., Ataker, F., Hedin, G., & Dornbusch, K. (2008). Molecular epidemiology of extended-spectrum -lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006. Journal of Clinical Microbiology, 46(2), 707-712.

Fashae, K., Engelmann, I., Monecke, S., Braun, S. D., & Ehricht, R. (2021). Molecular characterisation of extended-spectrum -lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria. BMC veterinary research, 17, 1-12.

Farzi, S., Ranjbar, R., Niakan, M., & Ahmadi, M. H. (2021). Molecular characterization of antibiotic resistance associated with TEM and CTX-M ESBL in uropathogenic E. coli strains isolated from outpatients. Iranian Journal of Pathology, 16(4), 386.

Gautam, V., Thakur, A., Sharma, M., Singh, A., Bansal, S., Sharma, A., . & Ray, P. (2019). Molecular characterization of extended-spectrum -lactamases among clinical isolates of Escherichia coli & Klebsiella pneumoniae: a multi-centric study from tertiary care hospitals in India. Indian Journal of Medical Research, 149(2), 208-215.

Giwa, F. J., Ige, O. T., Haruna, D. M., Yaqub, Y., Lamido, T. Z., & Usman, S. Y. (2018). Extended-spectrum beta-lactamase production and antimicrobial susceptibility pattern of uropathogens in a tertiary hospital in Northwestern Nigeria. Annals of Tropical Pathology, 9(1), 11-16.

Gujnsdttir, H. B. (2015). ESBL-producing Escherichia coli: a molecular study on bacterial relatedness and resistance genes in isolates from the period 2006-2014. Doctoral dissertation, University of Iceland.

Guiral Vilalta, E. (2018). Interplay of virulence, antibiotic resistance and epidemiology in Escherichia coli clinical isolates. Dissertation, Universitat de Barcelona.

Hardany, M. J., Al-Abdullah, A. A., Al-Amara, S. M., & Makki, H. M. (2019). Detection ESBL enzymes in gram-negative bacteria. Journal of Basrah Researches ((Sciences)), 45(2), 53-59.

Hassan, H., & Abdalhamid, B. (2014). Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital. The Journal of Infection in Developing Countries, 8(03), 282-288.

Ibrahim, D. R., Dodd, C. E., Stekel, D. J., Meshioye, R. T., Diggle, M., Lister, M., & Hobman, J. L. (2023). Multidrug-resistant ESBL-producing E. coli in clinical samples from the UK. Antibiotics, 12(1), 169.

Igwe, J. C., Onaolapo, J. A., Kachallah, M., Nworie, A., Oladipo, H. O., Ojiego, B. O., ... & Ibanga, I. A. (2014). Molecular characterization of extended spectrum -lactamase genes in clinical E. coli isolates. Journal of Biomedical Science and Engineering, 2014.

Iliyasu, M. Y., Uba, A., & Agbo, E. B. (2018). Phenotypic detection of multidrug resistant extended-spectrum beta-lactamase (ESBL) producing Escherichia coli from clinical samples. African Journal of Cellular Pathology, https://dx.doi.org/10(2),25-32.

Inwezerua, C. (2014). Molecular characterization of extended spectrum beta-lactamase in human and bovine Escherichia coli isolates. Dissertation, UI Postgraduate College.

Iroha, I. R., Esimone, C. O., Neumann, S., Marlinghaus, L., Korte, M., Szabados, F., ... & Kaase, M. (2012). First description of Escherichia coli producing CTX-M-15-extended spectrum beta lactamase (ESBL) in out-patients from south eastern Nigeria. Annals of Clinical Microbiology and Antimicrobials, 11, 1-5.

Khachab, Y., Hodroj, M., & Sokhn, E. S. (2025). Understanding and Addressing -Lactam Resistance Mechanisms in Gram-Negative Bacteria in Lebanon: A Scoping Review. Heliyon.

Kader, A. A., & Kumar, A. (2005). Prevalence and antimicrobial susceptibility of extended-spectrum -lactamase-producing Escherichia coli and Klebsiella pneumoniae in a general hospital. Annals of Saudi medicine, 25(3), 239-242.

Krishnamurthy, V., Vijaykumar, G. S., Kumar, S., Prashanth, H. V., Prakash, R., & Nagaraj, E. R. (2013). Phenotypic and genotypic methods for detection of extended spectrum lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from ventilator associated pneumonia. Journal of clinical and diagnostic research: JCDR, 7(9), 1975.

Mendes Pedro, D., Santos, D., Meneses, M., Gonalves, F., Domingos, G. J., & Caneiras, C. (2025). Risk of Colonization with Multidrug-Resistant Gram-Negative Bacteria Among Travellers and Migrants: A Narrative Review. Tropical Medicine and Infectious Disease, https://dx.doi.org/10(1),26.

Mohammed, Y., Gadzama, G. B., Zailani, S. B., & Aboderin, A. O. (2016). Characterization of extended-spectrum beta-lactamase from Escherichia coli and Klebsiella species from North Eastern Nigeria. Journal of clinical and diagnostic research: JCDR, 10(2), DC07.

Mohammed, A., Magashi, A. M., & Yushau, M. (2024). PHENOTYPIC DETECTION AND ANTIMICROBIAL SUSCEPTIBILITY PROFILE OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING Escherichia coli FROM SUSPECTED CASES OF URINARY TRACT INFECTION IN KANO METROPOLIS, NIGERIA. FUDMA JOURNAL OF SCIENCES, 8(2), 101-105.

Mohapatra, B. R., Broersma, K., & Mazumder, A. (2007). Comparison of five rep-PCR genomic fingerprinting methods for differentiation of fecal Escherichia coli from humans, poultry and wild birds. FEMS microbiology letters, 277(1), 98-106.

Mtimunye, M. F. (2018). Virulence capabilities of endemic bacteriophages against Colistin and Extended spectrum beta-lactam resistant non-O157 Escherichia coli strains. Doctoral dissertation, North-West University.

Obuekwe, I. S., & Ikediashi, H. C. (2021). PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF EXTENDED-SPECTRUM BETA-LACTAMASE PRODUCING Escherichia coli OBTAINED FROM ANIMAL FECAL MATERIALS IN OVIA NORTH-EAST, EDO STATE, NIGERIA. Nigerian Journal of Applied Sciences, 39, 10-20.

Olajide, O. E., Nwadike, B. I., Omotosho, O., & Oluwole, A. P. (2024). Presence and characterisation of extended spectrum beta-lactamase producing Gram-negative bacteria obtained from water in selected storage tanks. One Health Bulletin, 10-4103.

Odetoyin, B., & Adewole, O. (2021). Prevalence of Extended-Spectrum -Lactamase-Production among Clinical Gram-Negative Bacilli Isolates in A Tertiary Hospital, Southwest Nigeria. European Journal of Medical and Health Sciences, 3(5), 9-13.

Odewale, G., Jibola-Shittu, M. Y., Bala, H. E. M., Akogwu, R., & Raimi, L. O. (2024). ANTIBIOTIC SUSCEPTIBILITY AND DETECTION OF EXTENDED-SPECTRUM B-LACTAMASE GENES IN GRAM-NEGATIVE BACTERIA ISOLATED FROM AUTOMATED TELLER MACHINES. FUDMA JOURNAL OF SCIENCES, 8(1), 118-124.

Olowe, O. A., Adewumi, O., Odewale, G., Ojurongbe, O., & Adefioye, O. J. (2015). Phenotypic and molecular characterisation of extendedspectrum betalactamase producing Escherichia coli obtained from animal fecal samples in Ado Ekiti, Nigeria. Journal of Environmental and Public Health, 2015(1), 497980.

Olowe, O. A., Ayilara, O. A., Oladipo, G. O., Makanjuola, O. A., & Olaitan, J. O. (2012). Multidrug resistance Escherichia coli carrying extended-spectrum -lactamases enzymes in a tertiary care hospital in Osogbo, South Western Nigeria. Int J Pharma Med Biol Sci., pp.143-149.

Olowe, O. A., Okanlawon, B. M., Olowe, R. A., & Olayemi, A. B. (2008). Antimicrobial resistant pattern of Escherichia coli from human clinical samples in Osogbo, south western Nigeria. African Journal of Microbiology Research, 2(1), 8-11.

Onanuga, A., Mahindroo, J., Singh, S., & Taneja, N. (2019). Phenotypic and molecular characterization of antimicrobial resistant Escherichia coli from urinary tract infections in Port-Harcourt, Nigeria. Pan African Medical Journal, 34(1).

Or, P., Boonyayatra, S., Punyapornwithaya, V., & Awaiwanont, N. (2025). Prevalence of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Broiler Farms: A Systematic Review and Meta-Analysis: https://doi.org/10.12982/VIS.033. Veterinary Integrative Sciences, 23(2), 1-18.

Pandit, R., Awal, B., Shrestha, S. S., Joshi, G., Rijal, B. P., & Parajuli, N. P. (2020). Extendedspectrum Lactamase (ESBL) genotypes among multidrugresistant uropathogenic Escherichia coli clinical isolates from a Teaching Hospital of Nepal. Interdisciplinary perspectives on infectious diseases, 2020(1), 6525826.

Parajuli, N. P., Maharjan, P., Joshi, G., & Khanal, P. R. (2016). Emerging perils of extended spectrum -lactamase producing Enterobacteriaceae clinical isolates in a teaching hospital of Nepal. BioMed research international 2016(1):1782835. https://doi.org/10.1155/2016/1782835

Pearce, R., Pirolo, M., Goecke, N. B., Toppi, V., Good, L., Guitian, J., & Guardabassi, L. (2025). Imported seafood is a reservoir of Enterobacteriaceae carrying CTX-M-encoding genes of high clinical relevance. International Journal of Food Microbiology, 111063.

Pdia, U., Okoli, E. N., & Izomoh, I. M. (2006). Antimicrobial susceptibility and plasmid profiles of Escherichia coli isolates obtained from different human clinical specimens in LagosNigeria. J Am Sci, 2, 70-76.

Pokhrel, R. H., Thapa, B., Kafle, R., Shah, P. K., & Tribuddharat, C. (2014). Co-existence of beta-lactamases in clinical isolates of Escherichia coli from Kathmandu, Nepal. BMC Research Notes, 7, 1-5.

Sadeghi, M. R., Ghotaslou, R., Akhi, M. T., Asgharzadeh, M., & Hasani, A. (2016). Molecular characterization of extended-spectrum -lactamase, plasmid-mediated AmpC cephalosporinase and carbapenemase genes among Enterobacteriaceae isolates in five medical centres of East and West Azerbaijan, Iran. Journal of medical microbiology, 65(11), 1322-1331.

Saleh, Z. M., Michael, G., Adamu, M. T., Manga, M. M., & Garba, L. (2024). Molecular detection of extended-spectrum beta-lactamase (ESL)-encoding genes among clinical isolates of Enterobacteriaceae from Federal Teaching Hospital Gombe State Nigeria. Nigerian Journal of Biotechnology, 41(1), 175-190.

Sher, A. A., Whitehead-Tillery, C. E., Peer, A. M., Bell, J. A., Vocelle, D. B., Dippel, J. T., ... & Mansfield, L. S. (2025). Dynamic Spread of Antibiotic Resistance Determinants by Conjugation to a Human-Derived Gut Microbiota in a Transplanted Mouse Model. Antibiotics, 14(2), 152.

Skokov, A., Bogdanoviov, K., Kolkov, I., & Karpkov, R. (2015). Antimicrobial-resistant and extended-spectrum -Lactamaseproducing Escherichia coli in raw cow's milk. Journal of food protection, 78(1), 72-77.

Srisrattakarn, A., Saiboonjan, B., Tippayawat, P., Angkititrakul, S., Chanawong, A., Pornchoo, C., ... & Lulitanond, A. (2025). CTX-M, SHV, TEM and VEB -lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand. Journal of Infection and Public Health, 18(2), 102624.

Tabar, M. M., Mirkalantari, S., & Amoli, R. I. (2016). Detection of ctx-M gene in ESBL-producing E. coli strains isolated from urinary tract infection in Semnan, Iran. Electronic physician, 8(7), 2686.

Tanko, N., Bolaji, R. O., Olayinka, A. T., & Olayinka, B. O. (2020). A systematic review on the prevalence of extended-spectrum beta lactamase-producing Gram-negative bacteria in Nigeria. Journal of Global Antimicrobial Resistance, 22, 488-496.

Thonda, O., & Oluduro, A. (2017). Molecular characterization of ESBLs genes among multi-drug resistant Klebsiella species in Ile-Ife South-West, Nigeria. Journal of Advances in Microbiology, 7(2), 1-9.

Thonda, O. A., Oluduro, A. O., & Osuntokun, O. T. (2018). Prevalence of extended spectrum-lactamases in multidrug resistant strains of Gram-negative bacteria. African Journal of Microbiology Research, 12(7), 147-151..

Ugwu, M. C., Shariff, M., Nnajide, C. M., Beri, K., Okezie, U. M., Iroha, I. R., & Esimone, C. O. (2020). Phenotypic and molecular characterization of lactamases among enterobacterial uropathogens in southeastern Nigeria. Canadian Journal of Infectious Diseases and Medical Microbiology, 2020(1), 5843904.

Uyanga, F. Z., Ekundayo, E. O., Nwankwo, E. O., & Ibanga, I. A. (2020). Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria. World J Microbiol Biotechnol, 5(2), 48

Wilkie, E. D., Ezeani, C. V., Olasehinde, D. O., Oyedemi, O. T., Alao, J. O., Oluduro, A. O., & Oyetunde, O. B. (2024). Molecular analysis of multidrug-resistant E. coli in pediatric UTIs: findings from a Nigerian Hospital. The Journal of Infection in Developing Countries, 18(2), 1-8..

World Health Organization (WHO) (2018). E. coli. [Online]. https://www.who.int/news-room/fact-sheets/detail/e-coli

Zhou, J., Zhou, J., Chen, M., L, P., & Jiang, C. (2025). Prevalence of -lactam antibiotic resistance of Escherichia coli isolated from a neonatal intensive care unit. BMC pediatrics, 25(1), 95.

Published

19-08-2025

How to Cite

MOLECULAR PROFILING AND ANTIMICROBIAL RESISTANCE PATTERNS OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI ISOLATES FROM CLINICAL SAMPLES IN SOUTHWESTERN NIGERIA. (2025). FUDMA JOURNAL OF SCIENCES, 9(8), 274-285. https://doi.org/10.33003/fjs-2025-0908-3057

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Vol. 9 No. 8 (2025): FUDMA Journal of Sciences - Vol. 9 No. 8

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Research Articles
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FUDMA Journal of Sciences

How to Cite

MOLECULAR PROFILING AND ANTIMICROBIAL RESISTANCE PATTERNS OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI ISOLATES FROM CLINICAL SAMPLES IN SOUTHWESTERN NIGERIA. (2025). FUDMA JOURNAL OF SCIENCES, 9(8), 274-285. https://doi.org/10.33003/fjs-2025-0908-3057

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